Anti-Human EIF4E2 Polyclonal Antibody (HT124014)

Name: Anti-Human EIF4E2 Polyclonal Antibody
Brand: abinScience
SKU: HT124014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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개요

카탈로그 번호

HT124014

설명

Anti-Human EIF4E2 Polyclonal Antibody (HT124014) is a rabbit polyclonal antibody detecting EIF4E2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human EIF4E2 (Glu53-Pro245).

타겟

Eukaryotic translation initiation factor 4E type 2, eIF-4E type 2, eIF4E type 2, Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E-like 3, eIF4E-like protein 4E-LP, mRNA cap-binding protein 4EHP, h4EHP, mRNA cap-binding protein type 3, EIF4E2, EIF4EL3

정제

Purified by antigen affinity column.

등록번호

O60573

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Eukaryotic translation initiation factor 4E type 2 (EIF4E2) is a ~28 kDa protein. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation. Acts as a repressor of translation initiation. In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap. In P-bodies, component of a complex that promotes miRNA-mediated translational repression. Involved in virus-induced host response by mediating miRNA MIR34A-induced translational silencing which controls IFNB1 production by a negative feedback mechanism.

1. Rosettani, P. et al. (2007) Journal of molecular biology 368, 691-705. PMID: 17368478

2. Morita, M. et al. (2012) Molecular and cellular biology 32, 3585-93. PMID: 22751931

3. von Stechow, L. et al. (2015) Molecular and cellular biology 35, 1254-68. PMID: 25624349

4. Zhang, X. et al. (2021) Molecular cell 81, 1187-1199.e5. PMID: 33581076

5. Rom, E. et al. (1998) The Journal of biological chemistry 273, 13104-9. PMID: 9582349

6. Chapat, C. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 5425-5430. PMID: 28487484

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-Human EIF4E2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human EIF4E2 Polyclonal Antibody (HT124014)

개요

이미지

참고 문헌

데이터시트

SDS

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