Anti-Human ENDOG Polyclonal Antibody (HC488014)

Name: Anti-Human ENDOG Polyclonal Antibody
Brand: abinScience
SKU: HC488014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HC488014

설명

Anti-Human ENDOG Polyclonal Antibody (HC488014) is a rabbit polyclonal antibody detecting ENDOG in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ENDOG (Ala49-Lys297).

타겟

Endonuclease G mitochondrial, Endo G, EC:3.1.30.-, ENDOG

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

Q14249, P38447, O08600

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Endonuclease G, mitochondrial (ENDOG) is a ~32 kDa protein. Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA. The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs. Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs. Promotes conservative recombination when the DNA is 5hmC-modified. Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response.

1. Robertson, AB. et al. (2014) Nucleic acids research 42, 13280-93. PMID: 25355512

2. Wang, W. et al. (2021) Nature communications 12, 476. PMID: 33473107

3. Wiehe, RS. et al. (2018) Oncotarget 9, 18309-18326. PMID: 29719607

참고사항

For research use only

이미지

Western blot

Western blot analysis was performed using anti-ENDOG polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate

참고 문헌

데이터시트

Anti-Human ENDOG Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human ENDOG Polyclonal Antibody (HC488014)

개요

이미지

참고 문헌

데이터시트

SDS