Anti-Human ETF1 Polyclonal Antibody (HD118014)

Name: Anti-Human ETF1 Polyclonal Antibody
Brand: abinScience
SKU: HD118014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HD118014

설명

Anti-Human ETF1 Polyclonal Antibody (HD118014) is a rabbit polyclonal antibody detecting ETF1. Suitable for Human, Mouse, and Rat.

Highlights

●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ETF1 (Met1-Ser257).

타겟

ERF1, ETF1, Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, Protein Cl1, RF1, SUP45L1, TB3-1, eRF1

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

P62495

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

배경

Eukaryotic peptide chain release factor subunit 1 (ETF1) is a ~49 kDa protein. Component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons. The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. ETF1/ERF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA. Following GTP hydrolysis, eRF3 (GSPT1/ERF3A or GSPT2/ERF3B) dissociates, permitting ETF1/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.

1. Song, H. et al. (2000) Cell 100, 311-21. PMID: 10676813

2. Alkalaeva, EZ. et al. (2006) Cell 125, 1125-36. PMID: 16777602

3. Feng, T. et al. (2014) Molecular cell 53, 645-54. PMID: 24486019

4. Brown, A. et al. (2015) Nature 524, 493-496. PMID: 26245381

5. Shao, S. et al. (2016) Cell 167, 1229-1240.e15. PMID: 27863242

6. Oltion, K. et al. (2023) Cell 186, 346-362.e17. PMID: 36638793

7. Frolova, L. et al. (1994) Nature 372, 701-3. PMID: 7990965

8. Yamashita, A. et al. (2009) Genes & development 23, 1091-105. PMID: 19417104

9. Wang, X. et al. (2019) Cell 176, 625-635.e14. PMID: 30682371

참고사항

For research use only

이미지

참고 문헌

데이터시트

Anti-Human ETF1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human ETF1 Polyclonal Antibody (HD118014)

개요

이미지

참고 문헌

데이터시트

SDS