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Anti-Human G6PD Polyclonal Antibody (HY124014)

Anti-Human G6PD Polyclonal Antibody
Anti-Human G6PD Polyclonal Antibody
Anti-Human G6PD Polyclonal Antibody
Anti-Human G6PD Polyclonal Antibody
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사양:
  • 20ug
  • 50ug
  • 100ug
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  • 개요

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  • 데이터시트

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개요
카탈로그 번호HY124014
설명
Anti-Human G6PD Polyclonal Antibody (HY124014) is a rabbit polyclonal antibody detecting G6PD in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse, Rat
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human G6PD (Met1-Leu515).
타겟 Glucose-6-phosphate 1-dehydrogenase, G6PD
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 P11413
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Glucose-6-phosphate 1-dehydrogenase (G6PD) is a ~59 kDa protein. Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. Also catalyzes the conversion of NAADPH, which is produced by enzymes such as DUOX1, DUOX2 and NOX5 from NAADP and promotes Ca(2+) signaling during T cell activation, back to NAADP.

1. Gu, F. et al. (2021) Science signaling 14, eabe3800. PMID: 34784249
참고사항 For research use only.
이미지
  • Anti-Human G6PD Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-G6PD polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: LO2 cell lysate
    Lane 3: A431 cell lysate
    Lane 4: A549 cell lysate

  • Anti-Human G6PD Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-G6PD polyclonal antibody at 1ug/mL on mouse hippocampus.

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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