Anti-Human GABARAP Polyclonal Antibody (HF008014)

Name: Anti-Human GABARAP Polyclonal Antibody
Brand: abinScience
SKU: HF008014
Price: 168 USD
Availability: InStock

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개요
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SDS

개요

카탈로그 번호

HF008014

설명

Anti-Human GABARAP Polyclonal Antibody (HF008014) is a rabbit polyclonal antibody detecting GABARAP in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Cavia porcellus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human GABARAP (Met1-Leu117).

타겟

MM46, GABA(A) receptor-associated protein, GABARAP, Gamma-aminobutyric acid receptor-associated protein, FLC3B

정제

Purified by antigen affinity column.

등록번호

O95166

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Gamma-aminobutyric acid receptor-associated protein (GABARAP) is a ~13 kDa protein. Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. Also required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction. Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation.

1. Wang, H. et al. (1999) Nature 397, 69-72. PMID: 9892355

2. Kabeya, Y. et al. (2004) Journal of cell science 117, 2805-12. PMID: 15169837

3. Behrends, C. et al. (2010) Nature 466, 68-76. PMID: 20562859

4. Colecchia, D. et al. (2012) Autophagy 8, 1724-40. PMID: 22948227

5. Chino, H. et al. (2019) Molecular cell 74, 909-921.e6. PMID: 31006538

6. Genau, HM. et al. (2015) Molecular cell 57, 995-1010. PMID: 25684205

7. Lee, JH. et al. (2005) Biotechnology letters 27, 623-8. PMID: 15977068

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-Human GABARAP Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human GABARAP Polyclonal Antibody (HF008014)

개요

이미지

참고 문헌

데이터시트

SDS

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