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Anti-Human Galectin-3 Recombinant Antibody (M3/38) (HB167023)

Anti-Human Galectin-3 Recombinant Antibody (M3/38)
Anti-Human Galectin-3 Recombinant Antibody (M3/38)
Anti-Human Galectin-3 Recombinant Antibody (M3/38)
Anti-Human Galectin-3 Recombinant Antibody (M3/38)
Anti-Human Galectin-3 Recombinant Antibody (M3/38)
Anti-Human Galectin-3 Recombinant Antibody (M3/38)
Anti-Human Galectin-3 Recombinant Antibody (M3/38)
Anti-Human Galectin-3 Recombinant Antibody (M3/38)
Anti-Human Galectin-3 Recombinant Antibody (M3/38)
Anti-Human Galectin-3 Recombinant Antibody (M3/38)
Anti-Human Galectin-3 Recombinant Antibody (M3/38)
Anti-Human Galectin-3 Recombinant Antibody (M3/38)
Anti-Human Galectin-3 Recombinant Antibody (M3/38)
Anti-Human Galectin-3 Recombinant Antibody (M3/38)
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사양:
  • 20ug
  • 50ug
  • 100ug
  • 1mg
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  • 개요

  • 이미지

  • 참고 문헌

  • 데이터시트

  • SDS

개요
카탈로그 번호HB167023
종 반응성Human
응용 분야ELISA, FCM
숙주 종Mouse
클론성Monoclonal
이소타입IgG2a, kappa
클론 IDM3/38
타겟 Mac-2 antigen, Lectin L-29, LGALS3, Galactose-specific lectin 3, 35 kDa lectin, Galactoside-binding protein, CBP 35, L-31, Gal-3, Laminin-binding protein, Galectin-3, GALBP, IgE-binding protein, Carbohydrate-binding protein 35, MAC2
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
정제 Protein A/G purified from cell culture supernatant.
등록번호 P17931
RRID

Anti-Human Gal3/Galectin-3 Antibody (M3/38) (abinScience Cat# HB167023, RRID:AB_3729067)
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형태 Liquid
저장 완충액 0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:2000-1:20000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
배경

Galectin-3 (Gal-3) is a ~26 kDa protein. Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

참고사항 For research use only.
Isotype Control Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
이미지
  • Anti-Human Galectin-3 Recombinant Antibody (M3/38)

    Bioactivity

    Detects Human LGALS3 in indirect ELISAs.

  • Anti-Human Galectin-3 Recombinant Antibody (M3/38)

    Flow Cytometry

    Flow-cytometry using anti-human Galectin-3 antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog # HB167023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human Galectin-3 Recombinant Antibody (M3/38)

    Flow Cytometry

    Flow-cytometry using anti-human Galectin-3 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog # HB167023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human Galectin-3 Recombinant Antibody (M3/38)

    Flow Cytometry

    Flow-cytometry using anti-human Galectin-3 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog # HB167023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human Galectin-3 Recombinant Antibody (M3/38)

    Flow Cytometry

    Flow-cytometry using anti-human Galectin-3 antibody.SiHa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog # HB167023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human Galectin-3 Recombinant Antibody (M3/38)

    SDS-PAGE

    SDS-PAGE for Anti-Human Gal3/Galectin-3 Antibody (M3/38).

  • Anti-Human Galectin-3 Recombinant Antibody (M3/38)

    Flow cytometry

    Flow-cytometry using anti-human Galectin-3 antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 monoclonal antibody (Catalog HB167023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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