Anti-Human GCK Polyclonal Antibody (HW504014)

Name: Anti-Human GCK Polyclonal Antibody
Brand: abinScience
SKU: HW504014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HW504014

설명

Anti-Human GCK Polyclonal Antibody (HW504014) is a rabbit polyclonal antibody detecting GCK in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human GCK (Met1-Gln465).

타겟

Hexokinase-4, Hexokinase type IV, HK4, HK IV, Glucokinase, Hexokinase-D, GCK

정제

Purified by antigen affinity column.

등록번호

P35557

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Hexokinase-4 (GCK) is a ~52 kDa protein. Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant. Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues. Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell. In pancreas, plays an important role in modulating insulin secretion.

1. Christesen, HB. et al. (2002) Diabetes 51, 1240-6. PMID: 11916951

2. Cuesta-Muñoz, AL. et al. (2004) Diabetes 53, 2164-8. PMID: 15277402

3. Heredia, VV. et al. (2006) The Journal of biological chemistry 281, 40201-7. PMID: 17082186

4. Estalella, I. et al. (2008) Journal of human genetics 53, 460-466. PMID: 18322640

5. Pal, P. et al. (2009) Biochemistry 48, 814-6. PMID: 19146401

6. Raimondo, A. et al. (2014) Human molecular genetics 23, 6432-40. PMID: 25015100

7. Xu, LZ. et al. (1995) Biochemistry 34, 6083-92. PMID: 7742312

8. Takeda, J. et al. (1993) The Journal of biological chemistry 268, 15200-4. PMID: 8325892

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-Human GCK Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human GCK Polyclonal Antibody (HW504014)

개요

이미지

참고 문헌

데이터시트

SDS

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