中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HW371014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-Human HADHA Polyclonal Antibody (HW371014) is a rabbit polyclonal antibody detecting HADHA in ELISA, IHC, WB. Suitable for Human, Mouse, and Pig.
Highlights
|
||||||||
| 종 반응성 | Human | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human HADHA (Gly31-Gln763). | ||||||||
| 타겟 | 78 kDa gastrin-binding protein, HADH, HADHA, Trifunctional enzyme subunit alpha, mitochondrial, TP-alpha, Monolysocardiolipin acyltransferase | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P40939 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
||||||||
| 제품 사용 정보 |
| ||||||||
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Trifunctional enzyme subunit alpha, mitochondrial (HADHA) is a ~83 kDa protein. Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA described here carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities while the trifunctional enzyme subunit beta/HADHB bears the 3-ketoacyl-CoA thiolase activity. Independently of subunit beta, HADHA also exhibits a cardiolipin acyltransferase activity that participates in cardiolipin remodeling; cardiolipin is a major mitochondrial membrane phospholipid. 1. Carpenter, K. et al. (1992) Biochemical and biophysical research communications 183, 443-8. PMID: 1550553 2. Liang, K. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 7039-7044. PMID: 29915090 3. Xia, C. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 6069-6074. PMID: 30850536 4. Kamijo, T. et al. (1994) Biochemical and biophysical research communications 199, 818-25. PMID: 8135828 5. Miklas, JW. et al. (2019) Nature communications 10, 4671. PMID: 31604922 | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-HADHA polyclonal antibody at 1μg/ml on various samples.
Lane 1: THP-1 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: Mouse liver lysate
Lane 5: Mouse kidney lysate
Lane 6: Mouse heart lysate
Lane 7: Rat liver lysate
Lane 8: Rat kidney lysate
Lane 9: Rat heart lysate
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
