Anti-Human HLA-DRA*01 Polyclonal Antibody (HM812014)

Name: Anti-Human HLA-DRA*01 Polyclonal Antibody
Brand: abinScience
SKU: HM812014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HM812014

설명

Anti-Human HLA-DRA*01 Polyclonal Antibody (HM812014) is a rabbit polyclonal antibody detecting HLA-DRA in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human HLA-DRA (Ile26-Phe205).

타겟

major histocompatibility complex, class II, DR alpha, isoform CRA_a, HLA-DRA

내독소 수준

Please contact with the lab for this information.

순도

>95% as determined by SDS-PAGE.

정제

Purified by antigen affinity column.

등록번호

P01903

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

HLA class II histocompatibility antigen, DR alpha chain (HLA-DRA) is a ~28 kDa protein. An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells. Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins.

1. Shams, H. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 1966-77. PMID: 15265931

2. Röhn, TA. et al. (2004) Nature immunology 5, 909-18. PMID: 15322540

3. Deng, L. et al. (2007) Nature immunology 8, 398-408. PMID: 17334368

4. Kwok, WW. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 188, 2537-44. PMID: 22327072

5. Scally, SW. et al. (2013) The Journal of experimental medicine 210, 2569-82. PMID: 24190431

6. Muehling, LM. et al. (2016) Journal of immunology (Baltimore, Md. : 1950) 197, 3214-3224. PMID: 27591323

7. Galperin, M. et al. (2018) Science immunology 3. PMID: 29884618

8. Abelin, JG. et al. (2019) Immunity 51, 766-779.e17. PMID: 31495665

9. Stern, LJ. et al. (1994) Nature 368, 215-21. PMID: 8145819

10. Kropshofer, H. et al. (1997) Immunity 6, 293-302. PMID: 9075930

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-HLA-DRA polyclonal antibody at 1ug/mL on various samples.
Lane 1: Daudi cell lysate
Lane 2: Raji cell lysate

참고 문헌

데이터시트

Anti-Human HLA-DRA*01 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human HLA-DRA*01 Polyclonal Antibody (HM812014)

개요

이미지

참고 문헌

데이터시트

SDS