Anti-Human IKBKB Polyclonal Antibody (HT056014)

Name: Anti-Human IKBKB Polyclonal Antibody
Brand: abinScience
SKU: HT056014
Price: 96 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

20ug
50ug
100ug

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HT056014

설명

Anti-Human IKBKB Polyclonal Antibody (HT056014) is a rabbit polyclonal antibody detecting IKBKB in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human IKBKB (Glu16-Gly345).

타겟

IKKB, IKK-beta, Serine/threonine protein kinase IKBKB, I-kappa-B-kinase beta, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B kinase 2, IKBKB, IKK-B, NFKBIKB, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, IkBKB

정제

Purified by antigen affinity column.

등록번호

O14920

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Inhibitor of nuclear factor kappa-B kinase subunit beta (IKBKB) is a ~86 kDa protein. Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as a part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis.

1. Cui, J. et al. (2010) Cell 141, 483-96. PMID: 20434986

2. Tsuchiya, Y. et al. (2010) Molecular cell 39, 570-82. PMID: 20797629

3. Clark, K. et al. (2011) The Biochemical journal 434, 93-104. PMID: 21138416

4. Cardinez, C. et al. (2018) The Journal of experimental medicine 215, 2715-2724. PMID: 30337470

5. Mercurio, F. et al. (1997) Science (New York, N.Y.) 278, 860-6. PMID: 9346484

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-IKBKB polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate

참고 문헌

데이터시트

Anti-Human IKBKB Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human IKBKB Polyclonal Antibody (HT056014)

개요

이미지

참고 문헌

데이터시트

SDS

빠른 접근

문의하기