Anti-Human INTS8 Polyclonal Antibody (HA984014)

Name: Anti-Human INTS8 Polyclonal Antibody
Brand: abinScience
SKU: HA984014
Price: 168 USD
Availability: InStock

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100ug

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개요
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참고 문헌
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개요

카탈로그 번호

HA984014

설명

Anti-Human INTS8 Polyclonal Antibody (HA984014) is a rabbit polyclonal antibody detecting INTS8 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human INTS8 (Asn831-Phe995).

타겟

Integrator complex subunit 8, Int8, Protein kaonashi-1, C8orf52, INTS8

정제

Purified by antigen affinity column.

등록번호

Q75QN2

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Integrator complex subunit 8 (INTS8) is a ~113 kDa protein. Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes. The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA. The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs). Within the integrator complex, INTS8 is required for the recruitment of protein phosphatase 2A (PP2A) to transcription pause-release checkpoint.

1. Oegema, R. et al. (2017) PLoS genetics 13, e1006809. PMID: 28542170

2. Zheng, H. et al. (2020) Science (New York, N.Y.) 370. PMID: 33243860

3. Vervoort, SJ. et al. (2021) Cell 184, 3143-3162.e32. PMID: 34004147

4. Hu, S. et al. (2023) Molecular cell 83, 1588-1604.e5. PMID: 37080207

5. Fianu, I. et al. (2024) Nature 629, 219-227. PMID: 38570683

6. Baillat, D. et al. (2005) Cell 123, 265-76. PMID: 16239144

7. Huang, KL. et al. (2020) Molecular cell 80, 345-358.e9. PMID: 32966759

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human INTS8 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human INTS8 Polyclonal Antibody (HA984014)

개요

이미지

참고 문헌

데이터시트

SDS