Anti-Human LIG4 Polyclonal Antibody (HW824014)

Name: Anti-Human LIG4 Polyclonal Antibody
Brand: abinScience
SKU: HW824014
Price: 168 USD
Availability: InStock

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50ug
100ug

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HW824014

설명

Anti-Human LIG4 Polyclonal Antibody (HW824014) is a rabbit polyclonal antibody detecting LIG4 in ELISA, IHC, WB. Suitable for Human, Mouse, Cricetulus griseus, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human LIG4 (Met1-Lys609).

타겟

DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4, LIG4, DNA ligase 4

정제

Purified by antigen affinity column.

등록번호

P49917

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

DNA ligase 4 (LIG4) is a ~103 kDa protein. DNA ligase involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination. Catalyzes the NHEJ ligation step of the broken DNA during DSB repair by resealing the DNA breaks after the gap filling is completed. Joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. LIG4 is mechanistically flexible: it can ligate nicks as well as compatible DNA overhangs alone, while in the presence of XRCC4, it can ligate ends with 2-nucleotides (nt) microhomology and 1-nt gaps. Forms a subcomplex with XRCC4; the LIG4-XRCC4 subcomplex is responsible for the NHEJ ligation step and XRCC4 enhances the joining activity of LIG4.

1. Lee, JW. et al. (2003) Cancer research 63, 22-4. PMID: 12517771

2. Gu, J. et al. (2007) The EMBO journal 26, 1010-23. PMID: 17290226

3. Ochi, T. et al. (2013) Structure (London, England : 1993) 21, 672-9. PMID: 23523427

4. Kaminski, AM. et al. (2018) Nature communications 9, 2642. PMID: 29980672

5. Maddi, ER. et al. (2021) Protein engineering, design & selection : PEDS 34. PMID: 33586762

6. Robins, P. et al. (1996) The Journal of biological chemistry 271, 24257-61. PMID: 8798671

7. Grawunder, U. et al. (1997) Nature 388, 492-5. PMID: 9242410

8. Grawunder, U. et al. (1998) Molecular cell 2, 477-84. PMID: 9809069

9. Chen, L. et al. (2000) The Journal of biological chemistry 275, 26196-205. PMID: 10854421

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human LIG4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human LIG4 Polyclonal Antibody (HW824014)

개요

이미지

참고 문헌

데이터시트

SDS