Anti-Human LIMK1 Polyclonal Antibody (HW511014)

Name: Anti-Human LIMK1 Polyclonal Antibody
Brand: abinScience
SKU: HW511014
Price: 168 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

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50ug
100ug

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HW511014

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human LIMK1 (Gly419-Asp647).

타겟

LIMK-1, LIMK, LIMK1, LIM domain kinase 1

정제

Purified by antigen affinity column.

등록번호

P53667

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

LIM domain kinase 1 (LIMK1) is a ~72 kDa protein. Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation.

1. Maekawa, M. et al. (1999) Science (New York, N.Y.) 285, 895-8. PMID: 10436159

2. Niwa, R. et al. (2002) Cell 108, 233-46. PMID: 11832213

3. Kaji, N. et al. (2003) The Journal of biological chemistry 278, 33450-5. PMID: 12807904

4. Soosairajah, J. et al. (2005) The EMBO journal 24, 473-86. PMID: 15660133

5. Nishita, M. et al. (2005) The Journal of cell biology 171, 349-59. PMID: 16230460

6. Acevedo, K. et al. (2007) Experimental cell research 313, 4091-106. PMID: 18028908

7. Heng, YW. et al. (2012) Journal of cell science 125, 1579-90. PMID: 22328514

8. Borroni, EM. et al. (2013) Science signaling 6, ra30.1-11, S1-3. PMID: 23633677

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human LIMK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human LIMK1 Polyclonal Antibody (HW511014)

개요

이미지

참고 문헌

데이터시트

SDS

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