Anti-Human MAD2L2 Polyclonal Antibody (HP994014)

Name: Anti-Human MAD2L2 Polyclonal Antibody
Brand: abinScience
SKU: HP994014
Price: 96 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

20ug
50ug
100ug

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HP994014

설명

Anti-Human MAD2L2 Polyclonal Antibody (HP994014) is a rabbit polyclonal antibody detecting MAD2L2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human MAD2L2 (Met1-Ser211).

타겟

Mitotic arrest deficient 2-like protein 2, Mitotic spindle assembly checkpoint protein MAD2B, MAD2L2, MAD2-like protein 2, hREV7, REV7, REV7 homolog, MAD2B

정제

Purified by antigen affinity column.

등록번호

Q9UI95

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Mitotic spindle assembly checkpoint protein MAD2B (MAD2L2) is a ~24 kDa protein. Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs). During G1 and S phase of the cell cycle, the complex functions downstream of TP53BP1 to promote non-homologous end joining (NHEJ) and suppress DNA end resection.

1. Pfleger, CM. et al. (2001) Genes & development 15, 1759-64. PMID: 11459825

2. Chen, J. et al. (2001) Genes & development 15, 1765-70. PMID: 11459826

3. Zhang, L. et al. (2007) Molecular and cellular biology 27, 2861-9. PMID: 17296730

4. Iwai, H. et al. (2007) Cell 130, 611-23. PMID: 17719540

5. Hong, CF. et al. (2009) The Journal of biological chemistry 284, 19613-22. PMID: 19443654

6. Gupta, R. et al. (2018) Cell 173, 972-988.e23. PMID: 29656893

7. Hara, K. et al. (2010) The Journal of biological chemistry 285, 12299-307. PMID: 20164194

8. Xie, W. et al. (2012) Protein & cell 3, 864-74. PMID: 23143872

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-MAD2L2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: 293T cell lysate

참고 문헌

데이터시트

Anti-Human MAD2L2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human MAD2L2 Polyclonal Antibody (HP994014)

개요

이미지

참고 문헌

데이터시트

SDS

빠른 접근

문의하기