Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013)

Name: Anti-Human MAGEA3 Recombinant Antibody (SAA1562)
Brand: abinScience
SKU: HW798013
Price: 110 USD
Availability: InStock

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사양:

20ug
50ug
100ug
1mg

수량:

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HW798013

설명

Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013) is a mouse monoclonal antibody detecting MAGEA3 in ELISA, FCM. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, FCM

숙주 종

Mouse

클론성

Monoclonal

이소타입

IgG2a, kappa

클론 ID

SAA1562

테스트된 응용 분야

FCM

타겟

Antigen MZ2-D, MAGE3, Melanoma-associated antigen 3, CT1.3, MAGE-3 antigen, Cancer/testis antigen 1.3, MAGEA3

내독소 수준

< 10 EU/mg as determined by LAL method.

순도

>95% as determined by SDS-PAGE.

정제

Protein A/G purified from cell culture supernatant.

등록번호

P43357

RRID

Anti-Human MAGEA3 Antibody (SAA1562) (abinScience Cat# HW798013, RRID:AB_3727323)

형태

Liquid

저장 완충액

0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:2000-1:20000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

배경

Melanoma-associated antigen 3 (MAGEA3) is a ~34 kDa protein. Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a repressor of autophagy. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines.

1. Doyle, JM. et al. (2010) Molecular cell 39, 963-74. PMID: 20864041

2. Ravichandran, R. et al. (2019) EMBO reports 20, e47352. PMID: 31267705

3. Yang, B. et al. (2007) Cancer research 67, 9954-62. PMID: 17942928

4. Gaugler, B. et al. (1994) The Journal of experimental medicine 179, 921-30. PMID: 8113684

참고사항

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

이미지

SDS-PAGE

SDS-PAGE for Anti-Human MAGEA3 Antibody (SAA1562)
Flow cytometry

Flow-cytometry using anti-human MAGEA3 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MAGEA3 monoclonal antibody (Catalog HW798013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

참고 문헌

데이터시트

Anti-Human MAGEA3 Recombinant Antibody (SAA1562).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013)

개요

이미지

참고 문헌

데이터시트

SDS