Anti-Human MCM6 Polyclonal Antibody (HC528014)

Name: Anti-Human MCM6 Polyclonal Antibody
Brand: abinScience
SKU: HC528014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
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SDS

개요

카탈로그 번호

HC528014

설명

Anti-Human MCM6 Polyclonal Antibody (HC528014) is a rabbit polyclonal antibody detecting MCM6 in ELISA, IHC, WB. Suitable for Rat, Bovine, Human, Mouse, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human MCM6 (Arg190-Thr661).

타겟

DNA replication licensing factor MCM6, p105MCM, MCM6

정제

Purified by antigen affinity column.

등록번호

Q14566

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

DNA replication licensing factor MCM6 is a ~92 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

1. Tsuji, T. et al. (2006) Molecular biology of the cell 17, 4459-72. PMID: 16899510

2. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425

3. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004

4. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328

5. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

6. Ishimi, Y. (1997) The Journal of biological chemistry 272, 24508-13. PMID: 9305914

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-MCM6 polyclonal antibody at 1μg/ml on various samples.
Lane 1: HL60 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: PC12 cell lysate
Lane 6: NIH3T3 cell lysate
Lane 7: Mouse spleen lysate