Anti-Human MCM8 Polyclonal Antibody (HP407014)

Name: Anti-Human MCM8 Polyclonal Antibody
Brand: abinScience
SKU: HP407014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HP407014

설명

Anti-Human MCM8 Polyclonal Antibody (HP407014) is a rabbit polyclonal antibody detecting MCM8 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human MCM8 (Ser90-Lys366).

타겟

MCM8, C20orf154, Minichromosome maintenance 8, DNA helicase MCM8

정제

Purified by antigen affinity column.

등록번호

Q9UJA3

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

DNA helicase MCM8 is a ~93 kDa protein. Component of the MCM8-MCM9 complex, which is involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). The MCM8-MCM9 complex is a 3'-5' DNA helicase and single-stranded (ss)DNA-stimulated ATPase which binds ssDNA in the presence of nucleoside triphosphates. Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs, probably by regulating the localization of the MNR complex. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression.

1. Park, J. et al. (2013) Molecular and cellular biology 33, 1632-44. PMID: 23401855

2. McKinzey, DR. et al. (2023) Nucleic acids research 51, 7330-7341. PMID: 37309874

3. Lee, KY. et al. (2015) Nature communications 6, 7744. PMID: 26215093

4. Volkening, M. et al. (2005) Molecular and cellular biology 25, 1560-8. PMID: 15684404

5. Klucnika, A. et al. (2023) The Journal of cell biology 222. PMID: 36355348

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-Human MCM8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human MCM8 Polyclonal Antibody (HP407014)

개요

이미지

참고 문헌

데이터시트

SDS