Anti-Human METTL3 Polyclonal Antibody (HV489014)

Name: Anti-Human METTL3 Polyclonal Antibody
Brand: abinScience
SKU: HV489014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HV489014

설명

Anti-Human METTL3 Polyclonal Antibody (HV489014) is a rabbit polyclonal antibody detecting METTL3 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human METTL3 (Met1-Leu580).

타겟

METTL3, N6-adenosine-methyltransferase 70 kDa subunit, N6-adenosine-methyltransferase catalytic subunit, MTA70, Methyltransferase-like protein 3, MT-A70, hMETTL3

정제

Purified by antigen affinity column.

등록번호

Q86U44

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

N(6)-adenosine-methyltransferase catalytic subunit METTL3 is a ~64 kDa protein. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some RNAs and regulates various processes such as the circadian clock, differentiation of embryonic and hematopoietic stem cells, cortical neurogenesis, response to DNA damage, differentiation of T-cells and primary miRNA processing. In the heterodimer formed with METTL14, METTL3 constitutes the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability, processing, translation efficiency and editing. M6A acts as a key regulator of mRNA stability: methylation is completed upon the release of mRNA into the nucleoplasm and promotes mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs.

1. Dominissini, D. et al. (2012) Nature 485, 201-6. PMID: 22575960

2. Wang, X. et al. (2014) Nature 505, 117-20. PMID: 24284625

3. Liu, N. et al. (2015) Nature 518, 560-4. PMID: 25719671

4. Alarcón, CR. et al. (2015) Nature 519, 482-5. PMID: 25799998

5. Alarcón, CR. et al. (2015) Cell 162, 1299-308. PMID: 26321680

6. Meyer, KD. et al. (2015) Cell 163, 999-1010. PMID: 26593424

7. Wang, X. et al. (2016) Nature 534, 575-8. PMID: 27281194

8. Wang, P. et al. (2016) Molecular cell 63, 306-317. PMID: 27373337

9. Śledź, P. et al. (2016) eLife 5. PMID: 27627798

10. Xiang, Y. et al. (2017) Nature 543, 573-576. PMID: 28297716

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-METTL3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate

참고 문헌

데이터시트

Anti-Human METTL3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human METTL3 Polyclonal Antibody (HV489014)

개요

이미지

참고 문헌

데이터시트

SDS