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| 카탈로그 번호 | HV568014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-Human MLKL Polyclonal Antibody (HV568014) is a rabbit polyclonal antibody detecting MLKL in WB, IHC, ELISA. Suitable for Human.
Highlights
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| 종 반응성 | Human | ||||||||
| 응용 분야 | WB, IHC, ELISA | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human MLKL (Met1-Leu263). | ||||||||
| 타겟 | MLKL | ||||||||
| 내독소 수준 | Please contact with the lab for this information. | ||||||||
| 순도 | >90% as determined by SDS-PAGE. | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | Q8NB16 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 대체 이름 | Mixed lineage kinase domain-like protein, hMLKL, MLKL | ||||||||
| 배경 | Mixed lineage kinase domain-like protein (MLKL) is a ~54 kDa protein. Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following activation by ZBP1, MLKL is phosphorylated by RIPK3 in the nucleus, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function. 1. Sun, L. et al. (2012) Cell 148, 213-27. PMID: 22265413 2. Wang, Z. et al. (2012) Cell 148, 228-43. PMID: 22265414 3. Zhao, J. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 5322-7. PMID: 22421439 4. Cai, Z. et al. (2014) Nature cell biology 16, 55-65. PMID: 24316671 | ||||||||
| 참고사항 | For research use only. |
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