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| 카탈로그 번호 | HY454014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 종 반응성 | Human, Mouse, Rat | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human MTHFD1 (Met1-Lys297). | ||||||||
| 타겟 | MTHFC, C1-THF synthase, MTHFD1, C-1-tetrahydrofolate synthase, cytoplasmic, MTHFD | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P11586 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | C-1-tetrahydrofolate synthase, cytoplasmic (MTHFD1) is a ~101 kDa protein. Trifunctional enzyme that catalyzes the interconversion of three forms of one-carbon-substituted tetrahydrofolate: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate, 5,10-methenyltetrahydrofolate and (6S)-10-formyltetrahydrofolate. These derivatives of tetrahydrofolate are differentially required in nucleotide and amino acid biosynthesis, (6S)-10-formyltetrahydrofolate being required for purine biosynthesis while (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate is used for serine and methionine biosynthesis for instance. 1. Schmidt, A. et al. (2000) Biochemistry 39, 6325-35. PMID: 10828945 2. Christensen, KE. et al. (2009) Human mutation 30, 212-20. PMID: 18767138 3. Hum, DW. et al. (1991) Protein engineering 4, 493-500. PMID: 1881876 4. Burda, P. et al. (2015) Journal of inherited metabolic disease 38, 863-72. PMID: 25633902 | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-MTHFD1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: Hela cell lysate
Lane 5: A549 cell lysate
Lane 6: Mouse kidney lysate
Lane 7: Mouse liver lysate
Lane 8: Rat liver lysate
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