Anti-Human NBN Polyclonal Antibody (HF552014)

Name: Anti-Human NBN Polyclonal Antibody
Brand: abinScience
SKU: HF552014
Price: 168 USD
Availability: InStock

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100ug

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HF552014

설명

Anti-Human NBN Polyclonal Antibody (HF552014) is a rabbit polyclonal antibody detecting NBN in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human NBN (Met1-Asp121).

타겟

Nibrin, P95, NBS1, Cell cycle regulatory protein p95, NBS, Nijmegen breakage syndrome protein 1, NBN

정제

Purified by antigen affinity column.

등록번호

O60934

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Nibrin (NBN) is a ~84 kDa protein. Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination. Within the MRN complex, NBN acts as a protein-protein adapter, which specifically recognizes and binds phosphorylated proteins, promoting their recruitment to DNA damage sites.

1. Zhu, XD. et al. (2000) Nature genetics 25, 347-52. PMID: 10888888

2. Stiff, T. et al. (2005) The EMBO journal 24, 199-208. PMID: 15616588

3. Melander, F. et al. (2008) The Journal of cell biology 181, 213-26. PMID: 18411307

4. Chapman, JR. et al. (2008) EMBO reports 9, 795-801. PMID: 18583988

5. Wu, L. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 11200-5. PMID: 18678890

6. Yuan, J. et al. (2009) The Journal of biological chemistry 284, 31746-52. PMID: 19759395

7. Lu, CS. et al. (2012) The Journal of biological chemistry 287, 43984-94. PMID: 23115235

8. Rai, R. et al. (2017) Molecular cell 65, 801-817.e4. PMID: 28216226

9. Myler, LR. et al. (2017) Molecular cell 67, 891-898.e4. PMID: 28867292

10. Trujillo, KM. et al. (1998) The Journal of biological chemistry 273, 21447-50. PMID: 9705271

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human NBN Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human NBN Polyclonal Antibody (HF552014)

개요

이미지

참고 문헌

데이터시트

SDS