Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody (HP349014)

Name: Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody
Brand: abinScience
SKU: HP349014
Price: 96 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

20ug
50ug
100ug

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HP349014

설명

Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody (HP349014) is a rabbit polyclonal antibody detecting NEDD4.2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human NEDD4L/Nedd4-2 (Glu629-Asp975).

타겟

NEDD4L, E3 ubiquitin-protein ligase NEDD4-like, NEDD4.2, NEDL3, Nedd4-2, HECT-type E3 ubiquitin transferase NED4L, KIAA0439

정제

Purified by antigen affinity column.

등록번호

Q96PU5

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

E3 ubiquitin-protein ligase NEDD4-like (NEDD4L/Nedd4-2) is a ~111 kDa protein. E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Downregulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.

1. Kamadurai, HB. et al. (2009) Molecular cell 36, 1095-102. PMID: 20064473

2. Lee, DE. et al. (2020) Cell death & disease 11, 38. PMID: 31959741

3. Gao, P. et al. (2021) Nature communications 12, 1194. PMID: 33608556

4. Kuratomi, G. et al. (2005) The Biochemical journal 386, 461-70. PMID: 15496141

5. Nazio, F. et al. (2017) Cell cycle (Georgetown, Tex.) 16, 1744-1747. PMID: 28820317

6. Kang, Y. et al. (2015) The Biochemical journal 472, 71-82. PMID: 26363003

7. Anta, B. et al. (2016) The Journal of biological chemistry 291, 19132-45. PMID: 27445338

8. Low, LH. et al. (2015) The Journal of biological chemistry 290, 7141-50. PMID: 25631046

9. Yoo, JC. et al. (2013) Journal of dermatological science 72, 246-51. PMID: 23999003

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody (HP349014)

개요

이미지

참고 문헌

데이터시트

SDS

빠른 접근

문의하기