Anti-Human NEDD8 Polyclonal Antibody (HC254014)

Name: Anti-Human NEDD8 Polyclonal Antibody
Brand: abinScience
SKU: HC254014
Price: 168 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD): --

사양:

50ug
100ug

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HC254014

설명

Anti-Human NEDD8 Polyclonal Antibody (HC254014) is a rabbit polyclonal antibody detecting NEDD8. Suitable for Rat, Mouse, and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human NEDD8 (Leu2-Gln81).

타겟

NEDD-8, NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, Ubiquitin-like protein NEDD8

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

Q15843

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

배경

Ubiquitin-like protein NEDD8 is a ~9 kDa protein. Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53. Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M.

1. Liakopoulos, D. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 5510-5. PMID: 10318914

2. Hori, T. et al. (1999) Oncogene 18, 6829-34. PMID: 10597293

3. Amir, RE. et al. (2002) The Journal of biological chemistry 277, 23253-9. PMID: 11953428

4. Walden, H. et al. (2003) Molecular cell 12, 1427-37. PMID: 14690597

5. Xirodimas, DP. et al. (2004) Cell 118, 83-97. PMID: 15242646

6. Osaka, F. et al. (1998) Genes & development 12, 2263-8. PMID: 9694792

7. Horn-Ghetko, D. et al. (2024) Nature structural & molecular biology 31, 1083-1094. PMID: 38605244

8. Kokic, G. et al. (2024) Nature structural & molecular biology 31, 536-547. PMID: 38316879

참고사항

For research use only

이미지

참고 문헌

데이터시트

Anti-Human NEDD8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human NEDD8 Polyclonal Antibody (HC254014)

개요

이미지

참고 문헌

데이터시트

SDS