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| 카탈로그 번호 | HB870014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-Human NME1 Polyclonal Antibody (HB870014) is a rabbit polyclonal antibody detecting NME1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
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| 종 반응성 | Human, Mouse, Rat | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human NME1 (Met1-Glu152). | ||||||||
| 타겟 | NDPKA, NME1, NDP kinase A, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, NM23, Metastasis inhibition factor nm23, GAAD, Tumor metastatic process-associated protein, NDK A, NM23-H1 | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P15531 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Nucleoside diphosphate kinase A (NME1) is a ~17 kDa protein. Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis. Also phosphorylates geranyl pyrophosphate (GPP) and farnesyl pyrophosphate (FPP), linking it to isoprenoid metabolism. Additionally, functions as a non-specific serine/threonine kinase and histidine protein kinase, transferring phosphoryl groups from its active site to target proteins. May function as a Mg(2+)-dependent single-stranded DNA endonuclease as part of the SET complex, cooperating with the 3'-5' exonuclease TREX1 to mediate apoptotic DNA fragmentation in cytotoxic T lymphocytes. Reported to nick one DNA strand, enabling TREX1 to remove nucleotides from the free 3' end, enhancing DNA damage and suppressing DNA end reannealing and repair. 1. Wagner, PD. et al. (2000) The Journal of biological chemistry 275, 35570-6. PMID: 10952986 2. Ma, D. et al. (2004) The Journal of biological chemistry 279, 18073-84. PMID: 14960567 3. Yoon, JH. et al. (2005) Biochemistry 44, 15774-86. PMID: 16313181 4. Gilles, AM. et al. (1991) The Journal of biological chemistry 266, 8784-9. PMID: 1851158 5. Kim, MS. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 669-80. PMID: 23519676 6. Yu, BYK. et al. (2021) Redox biology 44, 101978. PMID: 33903070 7. MacDonald, NJ. et al. (1996) The Journal of biological chemistry 271, 25107-16. PMID: 8810265 8. Freije, JM. et al. (1997) The Journal of biological chemistry 272, 5525-32. PMID: 9038158 9. Engel, M. et al. (1995) European journal of biochemistry 234, 200-7. PMID: 8529641 | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-NME1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse brain lysate
Lane 2: Mouse lung lysate
Lane 3: Mouse liver lysate
Lane 4: Rat brain lysate
Lane 5: Rat lung lysate
Lane 6: Rat liver lysate
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