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Anti-Human NSUN5 Polyclonal Antibody (HP203014)

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개요
카탈로그 번호HP203014
설명
Anti-Human NSUN5 Polyclonal Antibody (HP203014) is a rabbit polyclonal antibody detecting NSUN5 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Human
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human NSUN5 (Met1-Arg429).
타겟 NOL1R, NOL1-related protein, NSUN5, 28S rRNA (cytosine-C(5))-methyltransferase, WBSCR20A, NOL1/NOP2/Sun domain family member 5, WBSCR20, NSUN5A, Williams-Beuren syndrome chromosomal region 20A protein
정제 Purified by antigen affinity column.
등록번호 Q96P11
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

28S rRNA (cytosine-C(5))-methyltransferase (NSUN5) is a ~46 kDa protein. S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 3782 (m5C3782) in 28S rRNA. m5C3782 promotes protein translation without affecting ribosome biogenesis and fidelity. Required for corpus callosum and cerebral cortex development.

1. Sharma, S. et al. (2013) Nucleic acids research 41, 9062-76. PMID: 23913415
2. Janin, M. et al. (2019) Acta neuropathologica 138, 1053-1074. PMID: 31428936
3. Heissenberger, C. et al. (2019) Nucleic acids research 47, 11807-11825. PMID: 31722427
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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