Anti-Human PCMT1 Polyclonal Antibody (HB868014)

Name: Anti-Human PCMT1 Polyclonal Antibody
Brand: abinScience
SKU: HB868014
Price: 96 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HB868014

설명

Anti-Human PCMT1 Polyclonal Antibody (HB868014) is a rabbit polyclonal antibody detecting PCMT1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human PCMT1 (Met1-Lys227).

타겟

PIMT, L-isoaspartyl protein carboxyl methyltransferase, PCMT1, Protein-L-isoaspartate(D-aspartate) O-methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase

정제

Purified by antigen affinity column.

등록번호

P22061

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT1) is a ~24 kDa protein. Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.

1. Gilbert, JM. et al. (1988) Biochemistry 27, 5227-33. PMID: 3167043

2. Murray, ED. et al. (1984) The Journal of biological chemistry 259, 10722-32. PMID: 6469980

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-PCMT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K562 cell lysate
Lane 5: Mouse testis lysate
Lane 6: Mouse heart lysate
Lane 7: Rat testis lysate
Lane 8: Rat heart lysate

참고 문헌

데이터시트

Anti-Human PCMT1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human PCMT1 Polyclonal Antibody (HB868014)

개요

이미지

참고 문헌

데이터시트

SDS