Anti-Human PHF5A Polyclonal Antibody (HA211014)

Name: Anti-Human PHF5A Polyclonal Antibody
Brand: abinScience
SKU: HA211014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HA211014

설명

Anti-Human PHF5A Polyclonal Antibody (HA211014) is a rabbit polyclonal antibody detecting PHF5A in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human PHF5A (Arg21-Arg110).

타겟

PHD finger-like domain-containing protein 5A, PHD finger-like domain protein 5A, Splicing factor 3B-associated 14 kDa protein, SF3b14b, PHF5A

정제

Purified by antigen affinity column.

등록번호

Q7RTV0

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

PHD finger-like domain-containing protein 5A (PHF5A) is a ~12 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, PHF5A is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs.

1. Will, CL. et al. (2002) The EMBO journal 21, 4978-88. PMID: 12234937

2. Cretu, C. et al. (2016) Molecular cell 64, 307-319. PMID: 27720643

3. Teng, T. et al. (2017) Nature communications 8, 15522. PMID: 28541300

4. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006

5. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human PHF5A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human PHF5A Polyclonal Antibody (HA211014)

개요

이미지

참고 문헌

데이터시트

SDS