Anti-Human PIK3CA Polyclonal Antibody (HW719014)

Name: Anti-Human PIK3CA Polyclonal Antibody
Brand: abinScience
SKU: HW719014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HW719014

설명

Anti-Human PIK3CA Polyclonal Antibody (HW719014) is a rabbit polyclonal antibody detecting PIK3CA. Suitable for Mouse, Rat, and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human PIK3CA (Asn345-Phe550).

타겟

EC:2.7.1.137, EC:2.7.1.153, EC:2.7.11.1, PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphoinositide 3-kinase alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, PtdIns-3-kinase subunit alpha, PtdIns-3-kinase subunit p110-alpha, Serine/threonine protein kinase PIK3CA, p110alpha

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

P42336

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

배경

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA) is a ~124 kDa protein. Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides. Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF.

1. Meier, TI. et al. (2004) Protein expression and purification 35, 218-24. PMID: 15135396

2. Buchanan, CM. et al. (2013) PloS one 8, e71337. PMID: 23936502

3. Maheshwari, S. et al. (2017) The Journal of biological chemistry 292, 13541-13550. PMID: 28676499

참고사항

For research use only

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참고 문헌

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Anti-Human PIK3CA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

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Anti-Human PIK3CA Polyclonal Antibody (HW719014)

개요

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참고 문헌

데이터시트

SDS

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