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| 카탈로그 번호 | HF629013 | ||||
|---|---|---|---|---|---|
| 종 반응성 | Human | ||||
| 응용 분야 | ELISA | ||||
| 숙주 종 | Mouse | ||||
| 클론성 | Monoclonal | ||||
| 이소타입 | IgG2a, kappa | ||||
| 클론 ID | SAA1537 | ||||
| 타겟 | RPA194, POLR1A, RNA polymerase I 194 kDa subunit, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit RPA1, RNA polymerase I subunit A1, A190, DNA-directed RNA polymerase I subunit A | ||||
| 내독소 수준 | Please contact with the lab for this information. | ||||
| 순도 | >95% as determined by SDS-PAGE. | ||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||
| 등록번호 | O95602 | ||||
| RRID | Anti-Human POLR1A Antibody (SAA1537) (abinScience Cat# HF629013, RRID:AB_3727298) |
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| 형태 | Liquid | ||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 배경 | DNA-directed RNA polymerase I subunit RPA1 (POLR1A) is a ~194 kDa protein. Catalytic core component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Transcribes 47S pre-rRNAs from multicopy rRNA gene clusters, giving rise to 5.8S, 18S and 28S ribosomal RNAs. Pol I-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol I pre-initiation complex (PIC) is recruited by the selectivity factor 1 (SL1/TIF-IB) complex bound to the core promoter that precedes an rDNA repeat unit. The PIC assembly bends the promoter favoring the formation of the transcription bubble and promoter escape. 1. Miller, G. et al. (2001) The EMBO journal 20, 1373-82. PMID: 11250903 2. Panov, KI. et al. (2001) Molecular and cellular biology 21, 2641-9. PMID: 11283244 3. Panov, KI. et al. (2006) The EMBO journal 25, 3310-22. PMID: 16858408 4. Zhao, D. et al. (2021) Cell discovery 7, 97. PMID: 34671025 5. Misiaszek, AD. et al. (2021) Nature structural & molecular biology 28, 997-1008. PMID: 34887565 6. Daiß, JL. et al. (2022) Life science alliance 5. PMID: 36271492 | ||||
| 참고사항 | For research use only. |
SDS-PAGE for Anti-Human POLR1A Antibody (SAA1537)
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