Anti-Human POLR3G Polyclonal Antibody (HT290014)

Name: Anti-Human POLR3G Polyclonal Antibody
Brand: abinScience
SKU: HT290014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
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SDS

개요

카탈로그 번호

HT290014

설명

Anti-Human POLR3G Polyclonal Antibody (HT290014) is a rabbit polyclonal antibody detecting POLR3G in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human POLR3G (Met1-Met110).

타겟

DNA-directed RNA polymerase III subunit RPC7, RNA polymerase III subunit C7, DNA-directed RNA polymerase III subunit G, RNA polymerase III 32 kDa apha subunit, RPC32-alpha, RNA polymerase III 32 kDa subunit, RPC32, POLR3G

정제

Purified by antigen affinity column.

등록번호

O15318

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

DNA-directed RNA polymerase III subunit RPC7 (POLR3G) is a ~25 kDa protein. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Acts as a long tether that bridges POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer and the mobile stalk of Pol III, coordinating the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state. Pol III exists as two alternative complexes defined by the mutually exclusive incorporation of subunit POLR3G/RPC7alpha or POLR3GL/RPC7beta. POLR3G/RPC7alpha modulates Pol III transcriptome by specifically enhancing the transcription of snaR-A non-coding RNAs.

1. Canella, D. et al. (2010) Genome research 20, 710-21. PMID: 20413673

2. Girbig, M. et al. (2021) Nature structural & molecular biology 28, 210-219. PMID: 33558764

3. Hou, H. et al. (2021) Nature communications 12, 6135. PMID: 34675218

4. Van Bortle, K. et al. (2022) Nature communications 13, 3007. PMID: 35637192

5. Haurie, V. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 4176-81. PMID: 20154270

6. Wang, Q. et al. (2021) Nature structural & molecular biology 28, 220-227. PMID: 33558766

7. Ablasser, A. et al. (2009) Nature immunology 10, 1065-72. PMID: 19609254

8. Chiu, YH. et al. (2009) Cell 138, 576-91. PMID: 19631370

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-POLR3G polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

참고 문헌

데이터시트

Anti-Human POLR3G Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human POLR3G Polyclonal Antibody (HT290014)

개요

이미지

참고 문헌

데이터시트

SDS

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