Anti-Human PRKACA Polyclonal Antibody (HB890014)

Name: Anti-Human PRKACA Polyclonal Antibody
Brand: abinScience
SKU: HB890014
Price: 168 USD
Availability: InStock

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사양:

50ug
100ug

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HB890014

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human PRKACA (His40-Lys310).

타겟

PRKACA, PKACA, PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha

정제

Purified by antigen affinity column.

등록번호

P17612

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

cAMP-dependent protein kinase catalytic subunit alpha (PRKACA) is a ~40 kDa protein. Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, histone H1.4 (H1-4), PSMC5/RPT6, PJA2, RYR2, RORA, SOX9, UHRF1 and VASP. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.

1. Guan, H. et al. (2005) The Journal of biological chemistry 280, 9957-62. PMID: 15642694

2. D'Souza, T. et al. (2005) The Journal of biological chemistry 280, 26233-40. PMID: 15905176

3. Pasqualucci, L. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 395-400. PMID: 16387847

4. Xu, H. et al. (2008) Breast cancer research and treatment 107, 63-70. PMID: 17333334

5. Zhang, F. et al. (2007) The Journal of biological chemistry 282, 22460-71. PMID: 17565987

6. Xiao, B. et al. (2007) The Journal of biological chemistry 282, 30256-64. PMID: 17693412

7. Shirakawa, K. et al. (2008) Nature structural & molecular biology 15, 1184-91. PMID: 18836454

8. Wang, W. et al. (2010) Molecular and cellular biochemistry 338, 115-22. PMID: 19949837

9. Gambaryan, S. et al. (2010) The Journal of biological chemistry 285, 18352-63. PMID: 20356841

10. Murshid, A. et al. (2010) PloS one 5, e13830. PMID: 21085490

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human PRKACA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human PRKACA Polyclonal Antibody (HB890014)

개요

이미지

참고 문헌

데이터시트

SDS

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