Anti-Human PRKRA Polyclonal Antibody (HF775014)

Name: Anti-Human PRKRA Polyclonal Antibody
Brand: abinScience
SKU: HF775014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HF775014

설명

Anti-Human PRKRA Polyclonal Antibody (HF775014) is a rabbit polyclonal antibody detecting PRKRA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human PRKRA (Asn126-Lys313).

타겟

PKR-associated protein X, Protein activator of the interferon-induced protein kinase, PACT, PRKRA, RAX, PKR-associating protein X, Interferon-inducible double-stranded RNA-dependent protein kinase activator A, Protein kinase, interferon-inducible double-stranded RNA-dependent activator

정제

Purified by antigen affinity column.

등록번호

O75569

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Interferon-inducible double-stranded RNA-dependent protein kinase activator A (PRKRA) is a ~34 kDa protein. Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner. May function as regulator of gastric epithelial differentiation.

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-PRKRA polyclonal antibody at 1μg/ml on various samples.
Lane 1: Rat testis lysate

참고 문헌

데이터시트

Anti-Human PRKRA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human PRKRA Polyclonal Antibody (HF775014)

개요

이미지

참고 문헌

데이터시트

SDS