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Anti-Human PSMB5 Polyclonal Antibody (HB987014)

Anti-Human PSMB5 Polyclonal Antibody
Anti-Human PSMB5 Polyclonal Antibody
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개요
카탈로그 번호HB987014
설명
Anti-Human PSMB5 Polyclonal Antibody (HB987014) is a rabbit polyclonal antibody detecting PSMB5 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse, Rat
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human PSMB5 (Gly59-Pro263).
타겟 MB1, PSMB5, Proteasome subunit MB1, X, Macropain epsilon chain, Proteasome subunit beta type-5, Multicatalytic endopeptidase complex epsilon chain, LMPX, Proteasome subunit X, Proteasome chain 6, Proteasome epsilon chain
정제 Purified by antigen affinity column.
등록번호 P28074
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Proteasome subunit beta type-5 (PSMB5) is a ~28 kDa protein. Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation.

참고사항 For research use only.
이미지
  • Anti-Human PSMB5 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PSMB5 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: LO2 cell lysate
    Lane 6: U2OS cell lysate
    Lane 7: K562 cell lysate
    Lane 8: PC-3 cell lysate
    Lane 9: MCF-7 cell lysate

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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