Anti-Human PUM1 Polyclonal Antibody (HD611014)

Name: Anti-Human PUM1 Polyclonal Antibody
Brand: abinScience
SKU: HD611014
Price: 168 USD
Availability: InStock

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사양:

50ug
100ug

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개요
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참고 문헌
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개요

카탈로그 번호

HD611014

설명

Anti-Human PUM1 Polyclonal Antibody (HD611014) is a rabbit polyclonal antibody detecting PUM1. Suitable for Human and Mouse.

Highlights

●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human PUM1 (Ser830-Ile1186).

타겟

HsPUM, KIAA0099, PUM1, PUMH1, Pumilio homolog 1, Pumilio-1

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

Q14671

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

배경

Pumilio homolog 1 (PUM1) is a ~126 kDa protein. Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle.

1. Gupta, YK. et al. (2008) Structure (London, England : 1993) 16, 549-57. PMID: 18328718

2. Lu, G. et al. (2011) Structure (London, England : 1993) 19, 361-7. PMID: 21397187

3. Filipovska, A. et al. (2011) Nature chemical biology 7, 425-7. PMID: 21572425

4. Dong, S. et al. (2011) The Journal of biological chemistry 286, 26732-42. PMID: 21653694

5. Van Etten, J. et al. (2012) The Journal of biological chemistry 287, 36370-83. PMID: 22955276

6. Galgano, A. et al. (2008) PloS one 3, e3164. PMID: 18776931

7. Kedde, M. et al. (2010) Nature cell biology 12, 1014-20. PMID: 20818387

8. Leibovich, L. et al. (2010) Silence 1, 17. PMID: 20860814

9. Miles, WO. et al. (2012) Genes & development 26, 356-68. PMID: 22345517

참고사항

For research use only

이미지

참고 문헌

데이터시트

Anti-Human PUM1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human PUM1 Polyclonal Antibody (HD611014)

개요

이미지

참고 문헌

데이터시트

SDS