Anti-Human RAN Polyclonal Antibody (HX918014)

Name: Anti-Human RAN Polyclonal Antibody
Brand: abinScience
SKU: HX918014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HX918014

설명

Anti-Human RAN Polyclonal Antibody (HX918014) is a rabbit polyclonal antibody detecting RAN in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human RAN (Met1-Asp171).

타겟

GTP-binding nuclear protein Ran, 3.6.5.-, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein, RAN, ARA24, OK/SW-cl.81

정제

Purified by antigen affinity column.

등록번호

P62826

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

GTP-binding nuclear protein Ran is a ~24 kDa protein. GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins.

1. Hsiao, PW. et al. (1999) The Journal of biological chemistry 274, 20229-34. PMID: 10400640

2. Yamashita, D. et al. (2007) The Journal of biological chemistry 282, 7563-75. PMID: 17209048

3. Rudack, T. et al. (2015) The Journal of biological chemistry 290, 24079-90. PMID: 26272610

4. Aksu, M. et al. (2016) Nature communications 7, 11952. PMID: 27306458

5. Melchior, F. et al. (1993) The Journal of cell biology 123, 1649-59. PMID: 8276887

6. Palacios, I. et al. (1996) The Journal of cell biology 133, 485-94. PMID: 8636225

7. Moroianu, J. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 7059-62. PMID: 8692944

8. Görlich, D. et al. (1996) The EMBO journal 15, 5584-94. PMID: 8896452

9. Izaurralde, E. et al. (1997) The EMBO journal 16, 6535-47. PMID: 9351834

10. Bischoff, FR. et al. (1997) FEBS letters 419, 249-54. PMID: 9428644

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human RAN Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human RAN Polyclonal Antibody (HX918014)

개요

이미지

참고 문헌

데이터시트

SDS