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Anti-Human RAP2A Polyclonal Antibody (HY078014)

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개요
카탈로그 번호HY078014
설명
Anti-Human RAP2A Polyclonal Antibody (HY078014) is a rabbit polyclonal antibody detecting RAP2A in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Human, Mouse, Rat
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human RAP2A (Met1-Gln183).
타겟 Ras-related protein Rap-2a, 3.6.5.2, RbBP-30, RAP2A
정제 Purified by antigen affinity column.
등록번호 P10114
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Ras-related protein Rap-2a (RAP2A) is a ~20 kDa protein. Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and regulates cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.

1. Machida, N. et al. (2004) The Journal of biological chemistry 279, 15711-4. PMID: 14966141
2. Taira, K. et al. (2004) The Journal of biological chemistry 279, 49488-96. PMID: 15342639
3. Mittal, V. et al. (2006) The Biochemical journal 394, 309-15. PMID: 16246175
4. Greco, F. et al. (2006) Biochimica et biophysica acta 1763, 330-5. PMID: 16540189
5. Nonaka, H. et al. (2008) Biochemical and biophysical research communications 377, 573-578. PMID: 18930710
6. Kawabe, H. et al. (2010) Neuron 65, 358-72. PMID: 20159449
7. Duncan, ED. et al. (2022) The Journal of cell biology 221. PMID: 35293963
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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