Anti-Human RBX1 Polyclonal Antibody (HX852014)

Name: Anti-Human RBX1 Polyclonal Antibody
Brand: abinScience
SKU: HX852014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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개요

카탈로그 번호

HX852014

설명

Anti-Human RBX1 Polyclonal Antibody (HX852014) is a rabbit polyclonal antibody detecting RBX1 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human RBX1 (Ala2-Arg46).

타겟

RING finger protein 75, RNF75, E3 ubiquitin-protein transferase RBX1, N-terminally processed, RBX1, E3 ubiquitin-protein ligase RBX1, Rbx1, ROC1, E3 ubiquitin-protein transferase RBX1, Protein ZYP, RING-box protein 1, Regulator of cullins 1

정제

Purified by antigen affinity column.

등록번호

P62877

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

E3 ubiquitin-protein ligase RBX1 is a ~12 kDa protein. E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex mediates ubiquitination of Pol II subunit POLR2A at 'Lys-1268', a critical TC-NER checkpoint. Core component of the Cul7-RING(FBXW8) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

1. Ohta, T. et al. (1999) Molecular cell 3, 535-41. PMID: 10230407

2. Kamura, T. et al. (1999) Genes & development 13, 2928-33. PMID: 10579999

3. Zheng, N. et al. (2002) Nature 416, 703-9. PMID: 11961546

4. Zhang, DD. et al. (2005) The Journal of biological chemistry 280, 30091-9. PMID: 15983046

5. Wang, H. et al. (2006) Molecular cell 22, 383-94. PMID: 16678110

6. Cen, B. et al. (2009) The Journal of biological chemistry 284, 5265-76. PMID: 19112177

7. Isobe, T. et al. (2009) The Journal of biological chemistry 284, 27766-27779. PMID: 19679664

8. Duda, DM. et al. (2012) Molecular cell 47, 371-82. PMID: 22748924

9. Beck, J. et al. (2013) Nature cell biology 15, 430-9. PMID: 23455478

10. Scott, DC. et al. (2016) Cell 166, 1198-1214.e24. PMID: 27565346

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-Human RBX1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human RBX1 Polyclonal Antibody (HX852014)

개요

이미지

참고 문헌

데이터시트

SDS