Anti-Human RFWD3 Polyclonal Antibody (HP698014)

Name: Anti-Human RFWD3 Polyclonal Antibody
Brand: abinScience
SKU: HP698014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HP698014

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human RFWD3 (Gly430-Glu774).

타겟

RING finger and WD repeat domain-containing protein 3, RNF201, E3 ubiquitin-protein ligase RFWD3, RING finger protein 201, RFWD3

정제

Purified by antigen affinity column.

등록번호

Q6PCD5

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

E3 ubiquitin-protein ligase RFWD3 is a ~85 kDa protein. E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signaling and repair. Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination. Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome.

1. Gong, Z. et al. (2011) The Journal of biological chemistry 286, 22308-13. PMID: 21504906

2. Liu, S. et al. (2011) The Journal of biological chemistry 286, 22314-22. PMID: 21558276

3. Elia, AE. et al. (2015) Molecular cell 60, 280-93. PMID: 26474068

4. Feeney, L. et al. (2017) Molecular cell 66, 610-621.e4. PMID: 28575657

5. Inano, S. et al. (2017) Molecular cell 66, 622-634.e8. PMID: 28575658

6. Gallina, I. et al. (2021) Molecular cell 81, 442-458.e9. PMID: 33321094

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-Human RFWD3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human RFWD3 Polyclonal Antibody (HP698014)

개요

이미지

참고 문헌

데이터시트

SDS

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