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| 카탈로그 번호 | HT159014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 종 반응성 | Human, Mouse | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human RIOK3 (Ser247-Glu519). | ||||||||
| 타겟 | Serine/threonine-protein kinase RIO3, SUDD, sudD homolog, RIO kinase 3, RIOK3 | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | O14730 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Serine/threonine-protein kinase RIO3 (RIOK3) is a ~59 kDa protein. Serine/threonine-protein kinase involved in a ribosome quality control that takes place when ribosomes have stalled, leading to 18S non-functional rRNA decay and degradation of the 40S ribosomal subunit. Acts downstream of RNF10: specifically recognizes and binds RPS2/us5 and RPS3/us3 monoubiquitinated by RNF10, promoting degradation of the 40S ribosomal subunit in a kinase-dependent manner. The RNF10-RIOK3 ribosome quality control takes place in response to ribosome subunit imbalance or downstream the EIF2AK4/GCN2-mediated integrated stress response (ISR). Also involved in regulation of type I interferon (IFN)-dependent immune response, possibly by acting as an adapter protein essential for the recruitment of TBK1 to IRF3. Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling. 1. Coria, AR. et al. (2025) Molecular cell 85, 787-801.e8. PMID: 39947182 2. Huang, Z. et al. (2025) Molecular cell 85, 802-814.e12. PMID: 39947183 3. Ford, PW. et al. (2025) Cell reports 44, 115371. PMID: 40022732 4. Feng, J. et al. (2014) Journal of virology 88, 7987-97. PMID: 24807708 | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-RIOK3 polyclonal antibody at 1µg/ml on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: Mouse testis lysate
Lane 5: Rat testis lysate
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