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Anti-Human RNF13 Polyclonal Antibody (HT135014)

Anti-Human RNF13 Polyclonal Antibody
Anti-Human RNF13 Polyclonal Antibody
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  • 개요

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개요
카탈로그 번호HT135014
설명
Anti-Human RNF13 Polyclonal Antibody (HT135014) is a rabbit polyclonal antibody detecting RNF13 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Human, Mouse, Rat
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human RNF13 (Glu33-Glu175).
타겟 E3 ubiquitin-protein ligase RNF13, 2.3.2.27, RING finger protein 13, RNF13, RZF
정제 Purified by antigen affinity column.
등록번호 O43567
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

E3 ubiquitin-protein ligase RNF13 is a ~42 kDa protein. E3 ubiquitin-protein ligase that regulates cell proliferation. Involved in apoptosis regulation. Mediates ER stress-induced activation of JNK signaling pathway and apoptosis by promoting ERN1 activation and splicing of XBP1 mRNA. Also involved in protein trafficking and localization.

1. Zhang, Q. et al. (2009) Cell research 19, 348-57. PMID: 18794910
2. Arshad, M. et al. (2013) The Journal of biological chemistry 288, 8726-8736. PMID: 23378536
3. Edvardson, S. et al. (2019) American journal of human genetics 104, 179-185. PMID: 30595371
4. van Dijk, JR. et al. (2014) The Biochemical journal 459, 27-36. PMID: 24387786
참고사항 For research use only.
이미지
  • Anti-Human RNF13 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-RNF13 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Rat brain lysate

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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