Anti-Human ROCK1 Polyclonal Antibody (HC470014)

Name: Anti-Human ROCK1 Polyclonal Antibody
Brand: abinScience
SKU: HC470014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HC470014

설명

Anti-Human ROCK1 Polyclonal Antibody (HC470014) is a rabbit polyclonal antibody detecting ROCK1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ROCK1 (Met1-Thr351).

타겟

Renal carcinoma antigen NY-REN-35, p160 ROCK-1, p160ROCK, Rho-associated protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, Rho-associated, coiled-coil-containing protein kinase 1, ROCK-I, ROCK1

정제

Purified by antigen affinity column.

등록번호

Q13464

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Rho-associated protein kinase 1 (ROCK1) is a ~158 kDa protein. Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability.

1. Maekawa, M. et al. (1999) Science (New York, N.Y.) 285, 895-8. PMID: 10436159

2. Ohashi, K. et al. (2000) The Journal of biological chemistry 275, 3577-82. PMID: 10652353

3. Sumi, T. et al. (2001) The Journal of biological chemistry 276, 670-6. PMID: 11018042

4. Sebbagh, M. et al. (2001) Nature cell biology 3, 346-52. PMID: 11283607

5. Hagerty, L. et al. (2007) The Journal of biological chemistry 282, 4884-4893. PMID: 17158456

6. Shao, J. et al. (2008) Molecular and cellular biology 28, 5196-208. PMID: 18573880

7. Wang, Y. et al. (2009) Circulation research 104, 531-40. PMID: 19131646

8. Ishizaki, T. et al. (1996) The EMBO journal 15, 1885-93. PMID: 8617235

9. Van Eyk, JE. et al. (1998) The Journal of biological chemistry 273, 23433-9. PMID: 9722579

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human ROCK1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human ROCK1 Polyclonal Antibody (HC470014)

개요

이미지

참고 문헌

데이터시트

SDS

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