Anti-Human RPL11 Polyclonal Antibody (HX025014)

Name: Anti-Human RPL11 Polyclonal Antibody
Brand: abinScience
SKU: HX025014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HX025014

설명

Anti-Human RPL11 Polyclonal Antibody (HX025014) is a rabbit polyclonal antibody detecting RPL11 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human RPL11 (Met1-Lys178).

타겟

60S ribosomal protein L11, CLL-associated antigen KW-12, Large ribosomal subunit protein uL5, RPL11

정제

Purified by antigen affinity column.

등록번호

P62913

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Large ribosomal subunit protein uL5 (RPL11) is a ~20 kDa protein. Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs.

1. Cmejla, R. et al. (2009) Human mutation 30, 321-7. PMID: 19191325

2. Liang, X. et al. (2020) Nature communications 11, 3542. PMID: 32669547

3. Odintsova, TI. et al. (2003) Journal of protein chemistry 22, 249-58. PMID: 12962325

4. Gazda, HT. et al. (2008) American journal of human genetics 83, 769-80. PMID: 19061985

5. Sloan, KE. et al. (2013) Cell reports 5, 237-47. PMID: 24120868

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-RPL11 polyclonal antibody at 1ug/mL on various samples.
Lane 1: BXPC-3 cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 5: NIH3T3 cell lysate
Lane 7: Mouse lung lysate
Lane 8: Mouse pancreas lysate
Lane 9: Rat liver lysate
Lane 10: Rat lung lysate
Lane11: Rat pancreas lysate

참고 문헌

데이터시트

Anti-Human RPL11 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human RPL11 Polyclonal Antibody (HX025014)

개요

이미지

참고 문헌

데이터시트

SDS

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