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Anti-Human SDHA Polyclonal Antibody (HB711014)

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개요
카탈로그 번호HB711014
설명
Anti-Human SDHA Polyclonal Antibody (HB711014) is a rabbit polyclonal antibody detecting SDHA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Danio rerio.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse, Rat
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human SDHA (Ser44-Tyr664).
타겟 SDH2, Flavoprotein subunit of complex II, Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial, SDHA, SDHF, Fp
정제 Purified by antigen affinity column.
등록번호 P31040
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA) is a ~72 kDa protein. Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate. Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate. Can act as a tumor suppressor.

1. Parfait, B. et al. (2000) Human genetics 106, 236-43. PMID: 10746566
2. Renkema, GH. et al. (2015) European journal of human genetics : EJHG 23, 202-9. PMID: 24781757
3. Burnichon, N. et al. (2010) Human molecular genetics 19, 3011-20. PMID: 20484225
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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