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Anti-Human SNX5 Polyclonal Antibody (HP226014)

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개요
카탈로그 번호HP226014
설명
Anti-Human SNX5 Polyclonal Antibody (HP226014) is a rabbit polyclonal antibody detecting SNX5 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse, Rat
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human SNX5 (Met1-Asp171).
타겟 SNX5, Sorting nexin-5
정제 Purified by antigen affinity column.
등록번호 Q9Y5X3
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Sorting nexin-5 (SNX5) is a ~46 kDa protein. Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable).

1. Merino-Trigo, A. et al. (2004) Journal of cell science 117, 6413-24. PMID: 15561769
2. van Weering, JR. et al. (2012) The EMBO journal 31, 4466-80. PMID: 23085988
3. Wassmer, T. et al. (2007) Journal of cell science 120, 45-54. PMID: 17148574
4. Hara, S. et al. (2008) Molecular biology of the cell 19, 3823-35. PMID: 18596235
5. Sun, Y. et al. (2013) Developmental cell 25, 144-55. PMID: 23602387
6. Schill, NJ. et al. (2014) Journal of cell science 127, 2189-203. PMID: 24610942
7. Lim, JP. et al. (2008) BMC cell biology 9, 58. PMID: 18854019
8. Wang, JT. et al. (2010) PloS one 5, e13763. PMID: 21048941
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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