Anti-Human SPOUT1 Polyclonal Antibody (HD297014)

Name: Anti-Human SPOUT1 Polyclonal Antibody
Brand: abinScience
SKU: HD297014
Price: 168 USD
Availability: InStock

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50ug
100ug

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HD297014

설명

Anti-Human SPOUT1 Polyclonal Antibody (HD297014) is a rabbit polyclonal antibody detecting SPOUT1. Suitable for Mouse and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human SPOUT1 (Thr74-Thr376).

타겟

28S rRNA (uridine-N(3))-methyltransferase, C9orf114, CENP-32, Centromere protein 32, EC:2.1.1.-, Kinetochore-associated protein, Methyltransferase C9orf114, Ribosomal RNA methyltransferase SPOUT1, SPOUT domain-containing methyltransferase 1, SPOUT1

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

Q5T280

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

배경

28S rRNA (uridine-N(3))-methyltransferase (SPOUT1) is a ~42 kDa protein. S-adenosyl-L-methionine-dependent RNA methyltransferase. Specifically methylates the N3 position of a uridine in 28S rRNA. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase, ensuring proper spindle organization. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis.

1. Chen, J. et al. (2024) Science advances 10, eadr1743. PMID: 39671501

2. Dharmadhikari, AV. et al. (2025) Nature communications 16, 1703. PMID: 39962046

3. Ohta, S. et al. (2010) Cell 142, 810-21. PMID: 20813266

4. Ohta, S. et al. (2015) Molecular biology of the cell 26, 1225-37. PMID: 25657325

5. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233

참고사항

For research use only

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참고 문헌

데이터시트

Anti-Human SPOUT1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human SPOUT1 Polyclonal Antibody (HD297014)

개요

이미지

참고 문헌

데이터시트

SDS