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| 카탈로그 번호 | HP128014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-Human STUB1 Polyclonal Antibody (HP128014) is a rabbit polyclonal antibody detecting STUB1 in ELISA, IHC, WB. Suitable for Chicken, Human, Mouse, and Rat.
Highlights
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| 종 반응성 | Human, Mouse, Rat | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human STUB1 (Ser23-Tyr303). | ||||||||
| 타겟 | Antigen NY-CO-7, CHIP, CLL-associated antigen KW-8, STUB1, Carboxy terminus of Hsp70-interacting protein, STIP1 homology and U box-containing protein 1, E3 ubiquitin-protein ligase CHIP, RING-type E3 ubiquitin transferase CHIP | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | Q9UNE7 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | E3 ubiquitin-protein ligase CHIP (STUB1) is a ~34 kDa protein. E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Plays a role in the maintenance of mitochondrial morphology and promotes mitophagic removal of dysfunctional mitochondria; thereby acts as a protector against apoptosis in response to cellular stress. Negatively regulates vascular smooth muscle contraction, via degradation of the transcriptional activator MYOCD and subsequent loss of transcription of genes involved in vascular smooth muscle contraction. Promotes survival and proliferation of cardiac smooth muscle cells via ubiquitination and degradation of FOXO1, resulting in subsequent repression of FOXO1-mediated transcription of pro-apoptotic genes. Ubiquitinates ICER-type isoforms of CREM and targets them for proteasomal degradation, thereby acts as a positive effector of MAPK/ERK-mediated inhibition of apoptosis in cardiomyocytes. 1. Ballinger, CA. et al. (1999) Molecular and cellular biology 19, 4535-45. PMID: 10330192 2. Connell, P. et al. (2001) Nature cell biology 3, 93-6. PMID: 11146632 3. Jiang, J. et al. (2001) The Journal of biological chemistry 276, 42938-44. PMID: 11557750 4. Matsumura, Y. et al. (2013) The Journal of biological chemistry 288, 31069-79. PMID: 23990462 5. Teng, Y. et al. (2015) Biochemical pharmacology 97, 518-530. PMID: 26265139 6. Li, F. et al. (2009) The Journal of biological chemistry 284, 20090-8. PMID: 19483080 7. Woo, CH. et al. (2010) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24, 4917-28. PMID: 20724525 8. Chao, CN. et al. (2019) Journal of cellular physiology 234, 20128-20138. PMID: 30980393 10. Parsons, JL. et al. (2009) The EMBO journal 28, 3207-15. PMID: 19713937 | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-STUB1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: L929 cell lysate
Lane 2: Hela cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: A549 cell lysate
Lane 5: PC-12 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Rat brain lysate
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