Anti-Human TAF1 Polyclonal Antibody (HB919014)

Name: Anti-Human TAF1 Polyclonal Antibody
Brand: abinScience
SKU: HB919014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
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SDS

개요

카탈로그 번호

HB919014

설명

Anti-Human TAF1 Polyclonal Antibody (HB919014) is a rabbit polyclonal antibody detecting TAF1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human TAF1 (Ser635-Gly944).

타겟

p250, TAF(II)250, CCGS, BA2R, TAF1, Transcription initiation factor TFIID subunit 1, Cell cycle gene 1 protein, TAFII-250, TAF2A, CCG1, TBP-associated factor 250 kDa, Transcription initiation factor TFIID 250 kDa subunit, TAFII250

정제

Purified by antigen affinity column.

등록번호

P21675

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Transcription initiation factor TFIID subunit 1 (TAF1) is a ~214 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. TAF1 is the largest component and core scaffold of the TFIID complex, involved in nucleating complex assembly. TAF1 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF2.

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473

2. Wang, H. et al. (2014) Cell research 24, 1433-44. PMID: 25412659

3. Louder, RK. et al. (2016) Nature 531, 604-9. PMID: 27007846

4. Dikstein, R. et al. (1996) Cell 84, 781-90. PMID: 8625415

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-TAF1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate