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Anti-Human TBP Polyclonal Antibody (HB847014)

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개요
카탈로그 번호HB847014
설명
Anti-Human TBP Polyclonal Antibody (HB847014) is a rabbit polyclonal antibody detecting TBP in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Human, Mouse
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human TBP (Ala155-Phe335).
타겟 TF2D, TATA-binding factor, GTF2D1, Transcription initiation factor TFIID TBP subunit, TFIID, TBP, TATA sequence-binding protein, TATA-box factor, TATA-box-binding protein
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 P20226, P29037
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

TATA-box-binding protein (TBP) is a ~37 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C. TBP forms the TFIID-A module together with TAF3 and TAF5.

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473
2. Kao, CC. et al. (1990) Science (New York, N.Y.) 248, 1646-50. PMID: 2194289
3. Peterson, MG. et al. (1990) Science (New York, N.Y.) 248, 1625-30. PMID: 2363050
4. Hoffman, A. et al. (1990) Nature 346, 387-90. PMID: 2374612
5. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682
6. Louder, RK. et al. (2016) Nature 531, 604-9. PMID: 27007846
참고사항 For research use only.
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참고 문헌
Formula
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Working Solution
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