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Anti-Human 5T4 Recombinant Antibody (SAA0148), APC (HC281137)

Anti-Human 5T4 Recombinant Antibody (SAA0148), APC
Anti-Human 5T4 Recombinant Antibody (SAA0148), APC
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  • 개요

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개요
카탈로그 번호HC281137
종 반응성Human
응용 분야FCM, IF, WB
숙주 종Mouse
클론성Monoclonal
이소타입IgG1, kappa
클론 IDSAA0148
접합APC
테스트된 응용 분야 FCM
타겟 5T4, TPBG, WAIF1, M6P1, 5T4 oncofetal trophoblast glycoprotein, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncotrophoblast glycoprotein, Wnt-activated inhibitory factor 1
내독소 수준 Please contact with the lab for this information.
관련 제품 Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), APC
등록번호 Q13641
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

안정성 및 저장 Store at 4°C for 12 months. Protect from light. Do not freeze.
배경

Trophoblast glycoprotein (TPBG/5T4) is a ~46 kDa protein. May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.

참고사항 For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
이미지
  • Anti-Human 5T4 Recombinant Antibody (SAA0148), APC

    Flow CytoMetry

    Flow-cytometry using APC anti-human ANGPT2 antibody. HUVEC cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human ANGPT2 monoclonal antibody (Catalog: HC281137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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