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| 카탈로그 번호 | HW726013 | ||||
|---|---|---|---|---|---|
| 종 반응성 | Human | ||||
| 응용 분야 | ELISA, IF, IP, IP-MS | ||||
| 숙주 종 | Mouse | ||||
| 클론성 | Monoclonal | ||||
| 이소타입 | IgG1 | ||||
| 클론 ID | SAA0237 | ||||
| 타겟 | Tuberous sclerosis 2 protein, TSC2, TSC4, Tuberin | ||||
| 내독소 수준 | Please contact with the lab for this information. | ||||
| 순도 | >95% as determined by SDS-PAGE. | ||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||
| 등록번호 | P49815 | ||||
| RRID | Anti-Human TSC2 IP Antibody (SAA0237) (abinScience Cat# HW726013, RRID:AB_3729771) |
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| 형태 | Liquid | ||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 배경 | Tuberin (TSC2) is a ~200 kDa protein. Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1. Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling. 1. Inoki, K. et al. (2002) Nature cell biology 4, 648-57. PMID: 12172553 2. Tee, AR. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 13571-6. PMID: 12271141 3. Castro, AF. et al. (2003) The Journal of biological chemistry 278, 32493-6. PMID: 12842888 4. Tee, AR. et al. (2003) Current biology : CB 13, 1259-68. PMID: 12906785 5. Li, Y. et al. (2004) Molecular and cellular biology 24, 7965-75. PMID: 15340059 6. Mazhab-Jafari, MT. et al. (2012) Structure (London, England : 1993) 20, 1528-39. PMID: 22819219 7. Menon, S. et al. (2014) Cell 156, 771-85. PMID: 24529379 8. Lim, JS. et al. (2017) American journal of human genetics 100, 454-472. PMID: 28215400 9. Yang, H. et al. (2021) Nature communications 12, 339. PMID: 33436626 10. Ali, ES. et al. (2022) Molecular cell 82, 3284-3298.e7. PMID: 35772404 | ||||
| 참고사항 | For research use only. |
SDS-PAGE for Anti-Human TSC2 IP Antibody (SAA0237)
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